Briefly, Met (0.21 mg/mL) and SN-38 (0.5 mg/mL) were dissolved in ethyl alcohol (ETOH) respectively to prepare stock solutions. Then 1 mL of each solution was injected into 100 mL DDW (with gentle agitation) at flow rate of 20 µL/min through two separated channels of syringe pump (LSP02-1B, Longer Pump Co., Ltd, Baoding, Hebei, China.). After the injection, reactions lasted for another 15 min. Afterward, the solution was centrifugated and obtained precipitate was dialyzed (MWCO 8000-14000) in DDW to completely remove ETOH and free drugs. The obtained MS NPs were stored in the dark at 4 °C before use.

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