The sample preparation and microarray hybridization were performed based on Agilent circRNA (Agilent Technologies Inc.). Briefly, total RNA from each sample was amplified and transcribed into fuorescent cRNA utilizing random primer according to Agilent circRNA protocol. The labeled cRNAs were hybridized onto the Agilent circRNA Array. After having washed the slides, the arrays were scanned by the Agilent scanner (G2565CA). Scanned images were then imported into Agilent Feature Extraction (v10.7) for grid alignment and data extraction. EdgeR package (R 3.4.4) was used to normalize the data and perform analysis to determine differential expression among the circRNAs.

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