The mRNA expressions and tumor subgroup gene expressions of TC2N in GC tissues and normal gastric tissues were analyzed using the sequencing data from the Cancer Genome Atlas (TCGA) database 23 by UALCAN database 24 and Gene Expression Profiling Interactive Analysis tools (GEPIA) 25. The LinkedOmics database 26 is a Web-based platform for analyzing 32 TCGA cancer-associated multi-dimensional datasets. The LinkFinder module of LinkedOmics was used to study genes differentially expressed in correlation with TC2N in the TCGA. Results were analyzed statistically using Spearman or Pearson's correlation coefficient. Protein-protein interaction (PPI) networks were predicted using the Gene MANIA 27 and STRING program 28.

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