RNA interference and Plasmid construction
This protocol is extracted from research article:
Overexpression of TC2N is associated with poor prognosis in gastric cancer
J Cancer, Jan 1, 2021; DOI: 10.7150/jca.50653

Two different sequences of TC2N siRNA, including negative control siRNA, were designed and chemically synthesized by Ribobio (China). These two siRNA candidates were listed as follows: si-001 (targeting GGATAAGTGAAGACAGTAA) and si-002 (targeting GCACGGACCTAGCTATGAT). The expression vector encoding full-length open reading frame of human TC2N were constructed by synthesis and inserted into p3xFLAG-CMV-14. SiRNAs or plasmid were transfected to cells with the Lipofectamine 3000 reagent (Invitrogen, USA) following the manufacturer protocol.

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