RNA interference and Plasmid construction
This protocol is extracted from research article:
Overexpression of TC2N is associated with poor prognosis in gastric cancer
J Cancer, Jan 1, 2021; DOI: 10.7150/jca.50653

Two different sequences of TC2N siRNA, including negative control siRNA, were designed and chemically synthesized by Ribobio (China). These two siRNA candidates were listed as follows: si-001 (targeting GGATAAGTGAAGACAGTAA) and si-002 (targeting GCACGGACCTAGCTATGAT). The expression vector encoding full-length open reading frame of human TC2N were constructed by synthesis and inserted into p3xFLAG-CMV-14. SiRNAs or plasmid were transfected to cells with the Lipofectamine 3000 reagent (Invitrogen, USA) following the manufacturer protocol.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.