Characterization of Immune Gene Signatures among the SKCM Immune clusters
This protocol is extracted from research article:
Decoding Immune Heterogeneity of Melanoma and identifying immune-prognostic hub genes
J Cancer, Jan 1, 2021; DOI: 10.7150/jca.50277

Cytolytic activity (CYT) was computed using an already validated gene expression signature using the geometric average of granzyme A (GZMA) and perforin-1 (PRF1) gene expression 17. Tumor Inflammation Signature (TIS) was quantified using the average of continuous mean of log2-transformed normalized expression of known genes 18. Relative antigen presentation machinery (APM) was quantified using a validated gene expression signature 19. Pathological assessment of the proportion of tumor infiltrating lymphocytes (TILs) 20 was also used.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.