Oncomine™ is a web-based data-mining platform consisting of multiple integrated cancer microarray databases and additional clinicopathological data 20. The hub gene-related clinical pathological data of tumors were searched for in this platform to explore the relationship between hub genes and aggressive biological behavior of tumors and analyzed in GraphPad Prism 8. Transcriptional expression of hub genes in 20 different types of cancer diseases was explored in Oncomine™ databases. Differences in transcriptional expression were compared by Student's t-test. The cutoff p value was 0.01; the cutoff fold change was 1.5; the gene rank was 10%, and data type was mRNA. Tumor pathological grade and stage of BC were investigated via the Curtis breast dataset (2136 cases), and metastatic event status of BC was studied via the Desmedt breast dataset (198 cases). The threshold of the p value was set as 1E-4, and the fold change was 2 in the dataset filter.

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