Three human PCa cell lines were used in this study: PC3, DU145, and LNCaP. PC3 and DU145 cell lines were obtained from the Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences. LNCaP cell line was acquired from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The basal medium was RPMI-1640 medium (Gibco) supplemented with 10% (v/v) fetal bovine serum (Gibco), 1% penicillin and 1% streptomycin (Invitrogen). LNCaP cells were cultured in basic medium supplemented with 1.0 mM L-glutamine and 1.0 mM sodium pyruvate (Invitrogen). All cells were incubated in a humidified incubator at 37°C and 5% CO2 (Thermo Scientific).

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