Statistical Program for Social Sciences (SPSS) 20.0 software (SPSS Inc., Chicago, IL, USA) and R 3.1.2 software (https://www.r-project.org/) were used to perform statistical analysis and to generate figures. Between-group comparisons of FOXD1 expression and the correlation between FOXD1 expression and clinicopathological features were performed using independent or paired Student's t-tests, as appropriate. Receiver operating characteristic curves were generated, and areas under the curves (AUC) were calculated to determine the diagnostic power of FOXD1 in HNSCC. The cut-off point was defined as the maximum Youden index. Pearson correlation coefficients were generated to evaluate the association between FOXD1 mRNA expression and FOXD1 DNA methylation levels. P ≤ 0.05 was considered statistically significant.

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