The Cancer Genome Atlas (TCGA)-Prostate adenocarcinoma (TCGA-PRAD) cohort data and the gene expression of 495 patients with PCa (499 prostate tissues) and 52 normal samples were obtained from the National Cancer Institute Genomic Data Commons (https://portal.gdc.cancer.gov/). R version 3.6.3 software was utilized to normalize and process the expression data. Oncomine database (https://www.oncomine.org/) is a powerful data-mining platform that contains 715 independent datasets and the data of 86,733 tumor tissues and adjacent normal tissues 24. In this study, we analyzed the mRNA expression levels of m6A regulators in different types of cancer by Oncomine data mining. The search parameters were as follows: type of analysis: cancer versus normal tissues; type of data: mRNA; P value: 0.05. The Human Cancer Metastasis Database (HCMDB, http://hcmdb.i-sanger.com/index) 25 is an online integrated database for storing and analyzing the large-scale expression data of cancer metastasis. We selected the data set of EXP00339 in HCMDB (dataset ID: GSE74685), which contains 89 CRPC samples that comprise 20 samples of bone metastasis and 69 of lymph node metastasis, to explore the expression of m6A regulators in CRPC with different metastasis sites.

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