Total RNA was extracted using Trizol (Code No. 9109, Takara Bio Inc, Japan) according to the manufacturer's instructions. cDNA was synthesized by reverse transcription using PrimeScript™ RT reagent Kit with gDNA Eraser (Code No. RR047A, Takara Bio Inc, Japan). qRT-PCR analysis was performed on a Roche LightCycler 480 II system according to the manufacturer's protocol. Primer sequences are listed in Table Table2.2. The housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control.

Primers used in this study

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