Human full-length DTX3 complementary DNA (cDNA) was constructed into pSlenti-EF1a-mcherry-P2A-Puro-CMV-DTX3-3Flag lentivirus vector. PTC cells were transfected with DTX3-cDNA or neg-vector, as the negative control for DTX3-cDNA, according to the manufacturer's protocols respectively (Obio Technology Co., Ltd, Shanghai, China). For stable transfection, PTC cells with puromycin resistance were selected (2.0 mg/mL for K1 and 3.0 mg/mL for TPC-1).

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