Gene function enrichment analysis, transcription factors, and cancer gene annotation

We used the R package clusterProfiler 15 to perform gene ontology and pathway enrichment analysis. Adjusted p-values less than 0.01 were obtained by the BH method were regarded as statistically significant. The human transcription factor list was downloaded from the supplementary file of Lambert et al. 16.

The oncogene list was obtained from the ONGene database 17 (http://ongene.bioinfo-minzhao.org/), and the tumor suppressor genes (TSGs) list was from the TSGene database 18 (https://bioinfo.uth.edu/TSGene/index.html). The activation of oncogenes and inactivation of TSGs may be associated with the cancer development.

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