The wild-type and mutant-type sequences of PAX5-3′-UTR were synthesized by GenePharma (Shanghai, China), and inserted into the pmiR-GLO dual-luciferase vector. Wild-type (WT) PAX5-3′-UTR and mutant-type (MUT) PAX5-3′-UTR were used to transfect cells that overexpressed miR-1254 or its control using Lipofectamine 2000 (Invitrogen, USA). Renilla luciferase expression plasmids were transfected into cells as a reference control. At 48 h, we used lysis buffer (Promega, USA) to lyse harvested cells. Firefly and Renilla luciferase activities were detected by the Dual-Luciferase Reporter Assay System (Promega, USA), according to the manufacturer's instructions.

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