The human HCC cell lines Hep3B, MHCC97-H, and Huh-7, and the human normal liver cell line LO2 were from the Chinese Academy of Sciences (Shanghai, China). All cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Life Technologies) and antibiotics (100 U/mL penicillin G and 100 mg/mL streptomycin) at 37°C in a humidified incubator with 5% CO2 atmosphere.

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