Also in the Article



SARS-CoV-2-IHUMI2, IHUMI3, IHUMI669, and IHUMI2123 strains were isolated from human nasopharyngeal swab as previously described [14] and used for all tests. The 4-passage strains were grown in VERO E6 (ATCC® CRL-1586™) before subculture in different cell lines in Minimum Essential Medium culture medium (Ref. 21090022, Thermo Fisher Scientific) with 4% fetal calf serum (Ref. 10270106, Thermo Fisher Scientific) and 1% L-glutamine (Ref. 25030024, Thermo Fisher Scientific), without antibiotics at 37 °C under 5% CO2.

Note: The content above has been extracted from a research article, so it may not display correctly.



Also in the Article

Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.