Peptide aptamer consists of two parts: a scaffold protein and a 5–20 residue peptide sequence binding to a particular target. Bacterial Thioredoxin A (UniProt ID P0AA25 and PDB ID 1KEB) was selected as a scaffold protein. The active site of Thioredoxin A consisting of the sequence CGPC was the point of insertion of the peptide aptamer. The SARS-CoV-2 spike protein binds to ACE2 through the receptor-binding domain. The region of ACE2 involved in binding to the SARS-CoV-2 receptor-binding domain was selected as an aptamer. The aptamer sequence is 18 residues long consisting of the sequence N-AKTFLDKFNHEAEDLFYQ-C and inserted between CGPC motifs of Thioredoxin A.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.