In order to achieve maximum expression in E. coli system, Jcat (Grote et al. 2005) was used for reverse translation and optimization of codons. Jcat is a rapid and easy method that could ensure maximum expression by calculating the GC content and CAI value (Grote et al. 2005). Restriction sites of BamHI and KpnI were added to the reverse translated sequence. The optimized sequence was then cloned into pET30a plasmid using Snapgene software.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.