The mouse-adapted H. pylori strain SS1 and other 12 clinical isolates strains H. pylori A-L were obtained from Shanghai Institute of Digestive Disease and then preserved in our laboratory. H. pylori was cultured on Columbia plates containing 7% newborn bovine serum and H. pylori selective supplement SR0147E (Oxoid, England) under microaerophilic conditions at 37 ℃ for 3–4 days.

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