Dengue IgM antibodies (DENV IgM) were detected using ELISA (NIV, Pune) if presentation was more than 3–5 days after the onset of clinical symptoms and/or NS1 antigen assay (Pan Bio, Queensland, Australia) if the presentation was within 3–5 days after the onset of clinical symptoms. Complete blood counts were analysed in the Institute’s emergency laboratory equipped with a Sysmex KX-21N automated hematology analyser from Sysmex Corporation, Kobe, Japan, which has a daily quality control check by the laboratory. Transaminases, serum urea and creatinine were analysed in the Institute’s central laboratory equipped with a Cobas 8000 analyser from Roche Hitachi Corporation, Japan.

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