Extracts (20–50 µg of protein) were examined by protein immunoblot analysis by probing with antibodies to AKT (Cell Signaling Technology 9272; RRID: AB_329827), pSer473 AKT (Cell Signaling Technology 9271; RRID: AB_329825), pThr308 AKT (Cell Signaling Technology 4056; RRID: AB_331163), JNK1/2 (BD Biosciences 554285, RRID: AB_395344), GAPDH (Santa Cruz Biotechnology sc-25778, RRID: AB_10167668), and αTubulin (clone B-5-1-2; Millipore-Sigma T5168; :RRID: 477579). IRDye 680LT-conjugated donkey anti-mouse immunoglobulin G (IgG) antibody (LI-COR Biosciences 926-68022, RRID: AB_10715072) and IRDye 800CW conjugated-goat anti-rabbit IgG (LI-COR Biosciences 926-32211, RRID: AB_621843) were used to detect and quantitate immune complexes with the Odyssey infrared imaging system (LI-COR Biosciences).

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