ALT and AST activity were determined in EDTA-treated plasma using the Alanine Transaminase Activity Assay Kit (ab105134) and the aspartate aminotransferase activity assay kit (ab138878) from Abcam. Both assays were performed following the manufacturer's recommendations. Samples were run together, analyzed in triplicate wells per mouse sample, and the mean value of these technical replicates was used for subsequent analysis.

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