Cells from synchronized embryos were dissociated into single cells by chitinase and mechanical shearing using syringe and 26G needle. RNA protein complexes were fixed with 0.15 J/cm2 of UV and complexes precipitated using GFP-Trap Dynabeads (chromotek) overnight at 4°C. After purification and digestion by Proteinase K RNA abundance was quantified by RT-qPCR.

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