Blood smear was prepared on microscope slides from blood films taken from the donor (infected) mouse tail. The smear was fixed with methanol and stained with Giemsa to count the parasitaemia of the donor under a microscope (Primo Star, Carl Zeiss, Germany). The mice were then inoculated on day 0 with parasitized erythrocytes obtained from the donor by cardiac puncture using a sterile syringe when the parasitaemia level was 30–40%. Blood from the donor was collected on a Petri dish containing 2% trisodium citrate and was immediately diluted with uninfected mouse blood and normal saline in such a way that the final volume contains 5 × 107 infected erythrocytes/ml of blood. The diluted blood (0.2 ml) was injected into all the experimental mice intraperitoneally (i.p) [25, 26].

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.