We computed and compared relative expression (means, fold changes and p-value) of the eight transcripts using Relative Expression Software Tool (REST)-384 version 2 software [44]. We then used these data to evaluate validity of the transcriptomes by comparing the fold changes obtained here (RT-qPCR) to those obtained earlier (RNA-Seq) for the eight genes through Pearson correlation analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.