Elemental analysis through energy dispersive X-ray spectroscopy (EDAX)

Considering the importance of source-sink status for determining the development of gall, the elemental complex analysis of galls in different developmental stages (n = 6) was carried out by using FE-SEM EDAX. Elemental analysis was performed using a ZEISS Gemini 300 FE-SEM, Germany. In total, 21 elements present in the gall samples were considered for analysis using EDAX. In the control leaves, elemental analysis was made based on vascular bundle and non-vascular tissue regions. But in the galls, owing to lack of perfect tissue organization, elemental analysis was done mainly based on the inner and outer regions of the galls.

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