We used the Seurat's FindAllMarkers function to identify marker genes for each cluster. This function contrasted cells from each cluster to all other cells of that cluster. The “RNA” assay and the “Wilcox” test were used for the function. Marker genes found by the FindAllMarkers function were required to have an average expression in that cluster that was >1.5‐fold higher than the average expression in the other clusters, and a detectable expression in >15% of cells from that cluster.

Marker genes of main cell types (Table S2), T‐cell subtypes (Table S3), and B‐cell subtypes (Table S4) were identified separately as described above.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.