The microplate reader was preheated 15 min before the measurement, and then the protein contents in BALF were evaluated in line with the instruction of the BCA kit (P0010, Beyotime Institute of Biotechnology, Shanghai, China). The optical density (OD) value at 570 nm was analyzed using the microplate reader. With the concentration of relative standard taken as the ordinate, the standard curve was phased, and the protein concentrations in BALF were calculated based on OD values of the samples by the standard curve.

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