Mice were intraperitoneally anesthetized by 1% pentobarbital sodium and fixed with a supine position (45°) to keep airway patency; then the tongue was moved to one side of the oral cavity. Next, the neck skin was disinfected and cut open to expose the trachea. The mixture of Exos or lentiviral vector was instilled into the trachea by a microsyringe, and the mice were held up and rotated after the instillation to make the mixture fully distribute in lung. The breath and heartbeat of the mice were observed during the instillation, which was immediately stopped if there appeared a cardiorespiratory arrest. After the trachea was unobstructed and the breath and heartbeat were stabilized, the instillation was continued.

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