The ALI mice models were established by cecal ligation puncture (CLP) [16]: the mice were fasted for 8 h before the surgery and then anesthetized by intraperitoneal injection of 1% pentobarbital sodium (80 mg/kg). Next, the mice were fixed with a supine position with their abdomen disinfected and with a longitudinal incision (1.0 cm) at the middle section of linea alba. The cecum was carefully taken out of the abdomen and 1/2 of the cecum was ligated by 1-0 suture, and the contralateral margin of mesentery was punched with some intestinal contents squeezed out, then the cecum was put back into the abdomen and the incision was sutured. Normal saline (1 mL) was subcutaneously injected into mice after surgery, and then the mice were normally fed in single cages.

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