Tibialis anterior muscles from 7-month-old mice were snap-frozen in isopentane cooled in liquid nitrogen and then stored at −80°C. Ten-micrometer-thick transverse sections were stained for glycerol-3-phosphate dehydrogenase (GPDH), succinate dehydrogenase (SDH), and NADH dehydrogenase (NADH tetrazolium reductase reaction, NADH-TR) activity as previously described (59). Livers were frozen in OCT embedding medium (Tissue-Tek). Ten-micrometer-thick transverse sections were fixed for 30 min in Baker solution (136 mM CaCl2 and 4% HCHO), rinsed with water, and then stained with Oil Red O (ORO) solution (12 mM ORO in 70% ethanol) for 5 min (liver) or 30 min (muscle). The adipose tissue from gonadal pads was fixed in 10% formalin for 48 hours and embedded in paraffin. Four-micrometer-thick transverse sections were stained with hematoxylin-eosin. Images were captured using an Axio Observer Z1 microscope (Zeiss) and analyzed in a blinded manner using the ImageJ software (v1.47, NIH).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.