The STORM optical instrument was built on a commercial inverted microscope base (Eclipse Ti-U with the perfect focus system, Nikon). The microscope is coupled to two imaging modalities. For STORM imaging, a 637-nm laser (Obis, Coherent) is collimated through a 100× 1.49 numerical aperture (NA) objective (SR APO TIRF, Nikon) with an average power at the sample of 3 to 10 kW/cm3. Images were collected via a 100× objective and sent to an electron-multiplying CCD (iXon Ultra 888, Andor). At least 8000 frames with a 20-ms acquisition time were collected from each sample. For PWS imaging, samples were illuminated with low NA light (0.5), and images are collected using the same 100× objective and sent through a liquid crystal tunable filter (LCTF; CRI VariSpec) and then to an sCMOS camera (ORCA Flash 4.0, Hamamatsu). The LCTF allows for spectrally resolved imaging. Images are collected between 500 and 700 nm with 2-nm intervals.

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