Confocal laser scanning microscopy
This protocol is extracted from research article:
Horizontal genome transfer by cell-to-cell travel of whole organelles
Sci Adv, Jan 1, 2021; DOI: 10.1126/sciadv.abd8215

Subcellular localization of dsRed and YFP fluorescence was determined by confocal laser scanning microscopy (TCS SP5 or TCS SP8, Leica, Wetzlar, Germany) using an argon laser for excitation (512 nm) and a 520- to 560-nm filter for detection of YFP fluorescence, a diode-pumped solid state laser at 561 nm for excitation, and a 575- to 603-nm filter for detection of dsRed fluorescence. Chlorophyll fluorescence was monitored by excitation with a helium-neon laser at 633 nm or a diode laser at 405 nm and detection with a 650- to 700-nm filter.

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