Multiplex PCR amplicons were subjected to library preparation and dual-indexing by using KAPA HyperPrep Kit and Unique Dual-Indexed Adaptor Kit (Roche) according to manufacturer’s instructions. Ligated libraries were enriched by 6-cycle PCR amplification and purification and size selection by using AMPure XP beads (Beckman Coulter, https://www.beckmancoulter.com/). The pooled library was sequenced with the MiSeq Reagent Kit V2 Nano on an Illumina MiSeq System (Illumina, https://www.illumina.com).

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