Multiplex PCR amplicons were subjected to library preparation and dual-indexing by using KAPA HyperPrep Kit and Unique Dual-Indexed Adaptor Kit (Roche) according to manufacturer’s instructions. Ligated libraries were enriched by 6-cycle PCR amplification and purification and size selection by using AMPure XP beads (Beckman Coulter, The pooled library was sequenced with the MiSeq Reagent Kit V2 Nano on an Illumina MiSeq System (Illumina,

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