We used the COVID-19 IgG ELISA developed by Mologic Ltd. and manufactured by Omega (Omega Diagnostics Group PLC, http://www.omegadiagnostics.com), according to the manufacturer’s instructions (Appendix 1). The assay contained the spike and nucleoprotein antigens of SARS-CoV-2. Between plate coefficients of variation were 21.0% (lower cutoff) and 16.5% (positive control; n = 16). Higher ambient temperatures in the laboratory resulted in higher optical density readings (Appendix 1).

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