We studied samples from patients in whom EVD was diagnosed during the 2013–2016 outbreak. We collected serum samples from 77 uninfected controls and 101 EVD survivors in Guéckédou, Guinea, during May 2015–September 2017. In addition, the European Mobile Laboratory provided DNA isolated from whole blood samples of 119 persons who had died of EVD.

We defined EVD survivors as EBOV-infected patients who had survived the acute phase of EVD and were discharged from the Ebola treatment center in Guéckédou after testing negative for Ebola 4 times by reverse transcription PCR. The survivors in our study included only persons with an original certificate of survivorship issued by the Guinean government. Controls tested negative for EBOV-specific antibodies and EBOV neutralizing antibodies in plasma.

We collected blood samples in EDTA tubes for routine blood tests, serologic assays for EBOV antigen, and nucleic acid detection of EBOV RNA by reverse transcription PCR. Afterward, we isolated peripheral blood mononuclear cells (PBMCs) from whole blood samples using Ficoll-Paque density gradient centrifugation (Sigma-Aldrich Inc., https://www.sigmaaldrich.com) according to the manufacturer’s instructions. To store PBMCs at room temperature, we used DNAgard Blood (Sigma-Aldrich Inc.) according to the manufacturer’s instructions.

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