Platanus Genome Assembler (v1.2.4)13 was used to assemble all the high-quality reads into scaffolds with parameters “-c 15 -k 60 -t 50 -m 300”. GapCloser (http://sourceforge.net/projects/soapdenovo2/files/GapCloser) was adopted with parameters “-p 25 -l 150” to fill gaps in the assembled scaffolds using PE reads. Read mapping, BUSCO, CEGMA, and BAC alignment were used to evaluate the quality of the assembled genome.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.