Leaf discs excised from different calla lily cultivars were prepared as previously described14, kept on half-strength Murashige and Skoog (MS) minimal medium, and challenged with 10 µl of bacterial suspension (108 CFU/ml, OD600 = 0.1) of Pb or distilled water as a control. The inoculated plant material was incubated at 28 °C. Disease progress was evaluated 24 h post inoculation, as the percentage of tissue decay relative to the total area of the leaf disc. The decayed area was measured using the Threshold_Colour plugin of the imageJ software (NIH, MD, USA). Three independent experiments were carried out, each using four leaves, with 10 replicates, 40 leaf discs per treatment (Pb/control for every cultivar). Fluorescence microscopy observations of the inoculated leaf discs were conducted as previously described33.

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