Four-week-old seedlings were used to determine the expression patterns of CmWRKY15-1 under different stress treatments. We sampled the leaves of cultivars C029 and Jinba at 0, 12, 24, 36, 48, and 72 h after treatment with P. horiana and at 0, 1, 6, 12, 24, and 48 h after treatment with 0.1 mM SA, 50 µM MeJA, 0.5 g L−1 ETH, and water. All the samples were stored at −80 °C, and each treatment was replicated three times. We quantified the relative expression levels of CmWRKY15-1 via quantitative real-time PCR (RT-qPCR), with CmActin used as the internal control (with the primer pair CmActin-F/R). We performed real-time qPCR according to the instructions provided with SYBR® Premix Ex Taq II. The PCR program was as follows: predenaturation at 95 °C for 30 s; 40 cycles of 95 °C for 5 s and 60 °C for 30 s; and a melt cycle of 95 °C for 15 s, 60 °C for 1 min, and 95 °C for 15 s. All the reactions were carried out three times for three independent biological replicates. We calculated the relative transcript levels of the target genes using the 2ΔΔCT method52. We set the expression level of CmWRKY15-1 in untreated leaves at 0–1 h for normalization across all the treatments.

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