The open reading frame (ORF) of SmMYB1 was cloned as described by Zhou et al.35. The primer pair used for gene cloning is listed in Supplementary Table S1. SmMYB1 was subjected to BLAST-Protein (BLASTP) analysis via the nonredundant (NR) protein sequence database ( Scutellaria taiwanensis StMYB13 (accession number AKA59794.1), Scutellaria indica SiMYB13 (AKA59777.1), and Sesamum indicum SiMYB5 (XP_011070362.1), which are highly homologous to SmMYB1 (MN400427), were subjected to amino acid sequence alignment using Vector NTI software (Invitrogen, USA). Sequence alignment and phylogenetic analysis were performed using ClustalX36. A phylogenetic tree was constructed based on amino acid sequences by the neighbor-joining method using MEGA 6.0 software, and the reliability of each node in the tree was evaluated using the bootstrap method, with 1000 replicates37.

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