Analysis of MSC proliferation was performed using BrdU labeling.24 MSCs were seeded onto 8-well chamber slides (Thermo Fisher Scientific, USA) at a concentration of 2 × 104 cells per well. After adherence, BrdU labeling reagent (Invitrogen, USA) was added to the medium at 1:100 for 48 h. The cells were then fixed with 70% ethanol, denatured with 2 N HCl, and stained with a BrdU Staining Kit (Invitrogen, USA) according to the manufacturer’s instructions. Fluoroshield mounting medium with DAPI (Abcam, UK) was used for counterstaining and mounting. The BrdU-positive cells in five fields of view from each sample were quantified using ImageJ software (National Institute of Health, USA) and are presented relative to the total number of cells.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.