Leaves and fruit from 8 ‘HoneySweet’ trees and 10 ‘Stanley’ trees located in various sites in Europe and the US were used to look at expression. RNAs were extracted from 20 mg of lyophilized leaf and fruit tissue using the Norgen Plant/Fungi Total RNA Purification Kit (Norgen Biotek Corp., ON) following the manufacturer’s protocol. The RNA was DNased using the TURBO DNA-free kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol. The RNA quality and purity were then assessed by electrophoresis on a 1.2% agarose gel and visualized on a Typhoon FLA9500 scanner (GE Healthcare Life Sciences, IL), and by analyzing spectrophotometrically on a NanoDrop® ND-1000 (Thermo Fisher Scientific, MA). Fruit and leaf RNA from each of the individual trees was sent to DHMRI for sequencing (36 libraries), unidirectional reads of 100-base lengths.

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