Leaves and fruit from 8 ‘HoneySweet’ trees and 10 ‘Stanley’ trees located in various sites in Europe and the US were used to look at expression. RNAs were extracted from 20 mg of lyophilized leaf and fruit tissue using the Norgen Plant/Fungi Total RNA Purification Kit (Norgen Biotek Corp., ON) following the manufacturer’s protocol. The RNA was DNased using the TURBO DNA-free kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol. The RNA quality and purity were then assessed by electrophoresis on a 1.2% agarose gel and visualized on a Typhoon FLA9500 scanner (GE Healthcare Life Sciences, IL), and by analyzing spectrophotometrically on a NanoDrop® ND-1000 (Thermo Fisher Scientific, MA). Fruit and leaf RNA from each of the individual trees was sent to DHMRI for sequencing (36 libraries), unidirectional reads of 100-base lengths.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.