Isolation and culture of MSCs from mouse bone marrow were performed according to our previous protocol.22,24 Briefly, whole bone marrow cells from femora and tibia were seeded, incubated overnight, and rinsed with phosphate-buffered saline (PBS) to remove the nonadherent cells. The adherent cells were cultured with alpha-minimum essential medium supplemented with 20% fetal bovine serum (FBS), 2 mmol·L−1 L-glutamine, 55 μmol·L1 2-mercaptoethanol, 100 U·mL−1 penicillin, and 100 μg·mL−1 streptomycin (all from Invitrogen, USA) at 37 °C in a humidified atmosphere of 5% CO2. MSCs were digested with 0.25% trypsin (Invitrogen, USA) and passaged for functional experiments after seeding at appropriate densities.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.