WT and Prx1-Cre;tdTomato mice at 8 weeks of age were subjected to continuous tail suspension for 2 weeks, which was performed according to previous studies.20 Briefly, the mice were individually caged and suspended by the tail using a strip of adhesive surgical tape attached to a chain hanging from a pulley. The mice were suspended at a 30° angle to the floor with only the forelimbs touching the floor, allowing the mice to move and access food and water freely. At sacrifice, the hindlimb bones were sampled for the indicated analyses.

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