Total RNA was extracted from fresh frozen liver by using the QIAamp RNA Blood Mini Kit (QIAGEN,, according to the manufacturer’s instructions. Amplification of YFV fragments were performed by using a described protocol (18) that targets the highly conserved 5′ noncoding region of the genome (112 bp) and is based on a TaqMan qRT-PCR protocol. Quantification cycle (Cq) was used as a reference for viral load (in a standard curve with YFV vaccine 17D, a titer of 105 PFU/mL had a mean Cq = 16, and 1 PFU/mL had a mean Cq = 35.6).

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