We performed statistical analysis using GraphPad Prism version 8.0.1 (https://www.graphpad.com) and IBM SPSS Statistics 23 (https://www.ibm.com/spss/statistics) software. We used linear regression analysis for numerical variables. The analysis of categorical variables was performed by Mann-Whitney test or 1-way analysis of variance (Kruskal-Wallis test) with Dunn’s correction for multiple comparisons, as appropriate. Two-sided p values <0.05 were considered significant.

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