The homogenized tissues were extracted for As compounds with 10 mM (NH4)2HPO4, and 30 mmol L–1 HNO3, centrifuged, filtered, and transferred to 1200 HPLC vessels (Agilent). An anion exchange separation column PRP-X100, 250 × 4.1 mm, 10 μm (Hamilton) and a guard column PRP-X100 20 × 2 mm, 10 μm (Hamilton) were used. The elution was performed isocratically at a flow rate of 1.0 mL min–1 (81). Calibration curves were obtained using stock solutions for As(III), As(V), DMA, and monomethylated As (MMA) in the mobile phase.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.