The homogenized tissues were extracted for As compounds with 10 mM (NH4)2HPO4, and 30 mmol L–1 HNO3, centrifuged, filtered, and transferred to 1200 HPLC vessels (Agilent). An anion exchange separation column PRP-X100, 250 × 4.1 mm, 10 μm (Hamilton) and a guard column PRP-X100 20 × 2 mm, 10 μm (Hamilton) were used. The elution was performed isocratically at a flow rate of 1.0 mL min–1 (81). Calibration curves were obtained using stock solutions for As(III), As(V), DMA, and monomethylated As (MMA) in the mobile phase.

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