The integration of the SEC-ICP-MS chromatograms was performed in the Origin X software package (OriginLab) after exporting them from the Agilent Mass Hunter ICP-MS software. The areas obtained were used to quantify the metal content of the SEC fraction and normalized to the starting tissue mass (80). The retention times for the molecular mass fraction were 10 to 19 minutes (> 500–10 kDa), 19.1–22 minutes (10–5 kDa), and 22.1–27 minutes (< 5 kDa).

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