Livers were obtained from KP-infected mice challenged with yRBCs or sRBCs 24 hours after KP infection. RNA was purified from liver tissue using RNeasy Plus Universal Mini kit (catalog 73407) according to the manufacturer’s instructions (Qiagen) and the concentration of isolated RNA was determined by NanoDrop. Purified RNA was sequenced using NextSeq 500 System (Illumina) at high output and paired-end read (2 × 150 cycles) by the Health Sciences Sequencing Core at Children’s Hospital of Pittsburgh. Sequencing data were analyzed with CLC Genomics Workbench (Qiagen). Briefly, the sequencing data quality was assessed, and low-quality reads (Phred quality score < 20) and adaptor sequences were trimmed for downstream analysis. RNA-Seq data were mapped against mouse genomic sequence, gene sequence, and mRNA sequence. Expression difference between treatment groups was considered significant when absolute fold change was greater than or equal to 1.5, maximum group mean was greater than or equal to 1, and the FDR P value was less than 0.05.

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