The median lobe of the liver was fixed in 2% paraformaldehyde for 4 hours. Subsequently, lobes were embedded in paraffin and processed for sectioning and H&E staining. Whole-section images of the median lobes at ×100 original magnification were obtained using a TissueFAXS PLUS system (Tissuegnostics). Using the overview images, the total numbers of inflammatory foci and necrotic regions in each liver section were assessed by an investigator blinded to experimental groups.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.