Tissues (15 mg) were homogenized in a solvent mixture of MeOH:ACN:H2O (50:30:20) in a TissueLyser II (Qiagen) at 4°C, followed by flash freezing in liquid nitrogen, and then centrifuged and passed through a Nanosep 3K molecular weight cutoff filter (Life Sciences Technologies). Tissue lysates were mixed 1:1 with 13C labeled internal standard mix balanced for the metabolites of interest. The balanced internal standard was generated by combining IROA yeast extract (IROA Technologies) with 13C-labeled lysates from several human cell lines grown in the presence of 5.5 mM U-13C glucose (Cambridge Isotopes) for 3 passages.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.